![]() Mahmood, I., Martinez-Hernandez, U., and Dehghani-Sanij, A.A.Tau, XMAP215/Msps and Eb1 co-operate interdependently to regulate microtubule polymerisation and bundle formation in axons. Hahn, I., Voelzmann, A., Parkin, J., Fülle, J.B., Slater, P.G., Lowery, L.A., Sanchez-Soriano, N., and Prokop, A.Activated I-BAR IRSp53 clustering controls the formation of VASP-actin–based membrane protrusions. Tsai, F.-C., Henderson, J.M., Jarin, Z., Kremneva, E., Senju, Y., Pernier, J., Mikhajlov, O., Manzi, J., Kogan, K., Le Clainche, C., et al.The x-axis represents the distance and the y-axis represents the time in the units set for the image for example pixel/time unit or micron/second. average speed - the average speed upto and including the current path segment.actual speed - the speed on the current path segment.dx now - the distance travelled on the current path segment in x direction.dy now - the distance travelled on the current path segment in y direction.dx sum - the sum of the distance travelled in x direction.dy sum - the sum of the distance travelled in y direction.The kymogram with a segmented line selection: Now make a segmented line selection on the trace of the particle in the kymogram and press the v-button to get the speed measurements. Now press the k-button to get the kymogram. You can use a z-projection (Image>Stacks>Z Project.) to create the selection and then transfer it to the time-series stack using ctrl+shift+e. Make a segmented line selection of the path of the particle for which you want to measure the velocity. The measurement of the velocities is a modified version of code from (tsp050706.txt TimeSpacePlot (Kymograph)) by J. ![]() ![]() the v-button measures the velocities corresponding to the line or segmented line selection on the kymogram.the k-button creates a kymogram of the current image.Select the "Velocity Measurement Tool" toolset from the > button of the ImageJ launcher. To install the tools, drag the link Velocity_Measurement_Tool.txt to the ImageJ launcher window, save it under macros/toolsets in the ImageJ installation and restart ImageJ. A synthetic example image can be found here: moving-particles-2.tif Getting started ¶ The user can make a segmented line selection on the kymogram and the mean velocity for each segment will be measured. The toolset allows to create kymograms and to measure the velocity of particles in the kymogram. ![]()
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